皮肤对胆红素的摄取与释放
Uptake and release of bilirubin by skin.
作者信息
Kapoor C L, Murti C R, Bajpai P C
出版信息
Biochem J. 1973 Sep;136(1):35-43. doi: 10.1042/bj1360035.
Abstract
- Skin epithelium of albino rat, mouse and guinea pig was shown to accumulate bilirubin from a medium containing free or bound bilirubin. 2. The K(m) values for bound bilirubin were 2.22x10(-3), 1.33x10(-3) and 9.5x10(-4)m for rat, mouse and guinea pig respectively and the corresponding K(m) values for free bilirubin were 5.2x10(-4), 4.0x10(-4), 1.8x10(-4)m; the V(max.) values of bound and free bilirubin were unchanged. 3. The uptake showed saturation kinetics. Bound bilirubin was released together with serum proteins. Free bilirubin bound to skin was not released into the medium. 4. Freezing and thawing of skin epithelium did not cause any significant lowering of the uptake of bilirubin but heat-denatured skin epidermis took up only 50% of the bound bilirubin or free bilirubin taken up by control unheated skin epithelium. 5. The uptake of free and bound bilirubin was prevented by HgCl(2) but not by sodium arsenate, NaCN, NaF, cycloheximide, 2,4-dinitrophenol or iodoacetate. 6. Most of the free bilirubin was bound to the lipids or lipoprotein fraction of skin epithelium and could be extracted by solvents. 7. Rat skin showed the highest accumulation and efflux of bilirubin.
摘要
- 已证明白化大鼠、小鼠和豚鼠的皮肤上皮能够从含有游离胆红素或结合胆红素的培养基中积累胆红素。2. 大鼠、小鼠和豚鼠结合胆红素的米氏常数（K(m)）分别为2.22×10⁻³、1.33×10⁻³和9.5×10⁻⁴m，游离胆红素的相应米氏常数分别为5.2×10⁻⁴、4.0×10⁻⁴、1.8×10⁻⁴m；结合胆红素和游离胆红素的最大反应速度（V(max.)）值未发生变化。3. 摄取呈现饱和动力学。结合胆红素与血清蛋白一起释放。与皮肤结合的游离胆红素不会释放到培养基中。4. 皮肤上皮的冻融不会导致胆红素摄取量显著降低，但热变性的皮肤表皮摄取的结合胆红素或游离胆红素仅为对照未加热皮肤上皮摄取量的50%。5. HgCl₂可阻止游离胆红素和结合胆红素的摄取，但砷酸钠、NaCN、NaF、环己酰亚胺、2,4-二硝基苯酚或碘乙酸则不能。6. 大部分游离胆红素与皮肤上皮的脂质或脂蛋白部分结合，可被溶剂提取。7. 大鼠皮肤显示出最高的胆红素积累和流出。
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