对来自受辐照人类胚胎细胞培养物的分离克隆群体的研究。
Studies on isolated cloned populations from irradiated human embryonic cell cultures.
作者信息
Lee C L, Lee S H, Kamra O P
出版信息
Cytogenet Cell Genet. 1979;24(3):150-9. doi: 10.1159/000131371.
The recovery of substrains with stable chromosome aberrations from irradiated fibroblast culture are reported. Four human fetal cell strains were exposed to 600 rad of gamma rays at 200 rad/min. The efficiency of recovering viable cloned subpopulations was approximately 87%, and the frequency of clones with abnormal chromosomes was 40/100 colonies. G-band chromosome analyses for 34 abnormal substrains are described. Karyotypes of some of the clones with complex rearrangements are also presented. Analyses of a total of 47 aberrant events in the 34 abnormal substrains revealed at 7:1 and a 9:1 translocation-inversion and translocation-deletion ratios, respectively. Five of the abnormal substrains were continuously cultured; all except one showed signs of sensecence toward the end of 44 +/- 10 doublings. Unusual prolonged proliferation capacity was observed in substrain FFS-1-9. The significance of this finding is discussed.
报道了从经辐照的成纤维细胞培养物中获得具有稳定染色体畸变的亚株的情况。四株人胎儿细胞株以200拉德/分钟的剂量接受600拉德的γ射线照射。回收存活克隆亚群的效率约为87%，染色体异常的克隆频率为40/100个集落。描述了对34个异常亚株的G带染色体分析。还展示了一些具有复杂重排的克隆的核型。对34个异常亚株中总共47个异常事件的分析分别显示易位-倒位和易位-缺失的比例为7:1和9:1。对五个异常亚株进行了连续培养；除一个外，所有亚株在44±10次倍增结束时均表现出衰老迹象。在亚株FFS-1-9中观察到了异常延长的增殖能力。讨论了这一发现的意义。
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