蝗虫脂肪体磷酸化酶的调节
Regulation of locust fat-body phosphorylase.
作者信息
Applebaum S W, Schlesinger H M
出版信息
Biochem J. 1973 Sep;135(1):37-41. doi: 10.1042/bj1350037.
Abstract
- Glycogen phosphorylase of locust fat-body was partially purified by differential centrifugation and dissociation from glycogen particles at two pH values. 2. Optimum activity was obtained at pH6.6-6.7. 3. The calculated apparent K(m) values for glycogen and glucose 1-phosphate were 0.08% and 10-13mm respectively. 4. 5'-AMP activated in the range 5mum-1mm. 5. Glucose 6-phosphate is a competitive inhibitor for the substrate glucose 1-phosphate (K(i)=1.7mm). 5'-AMP abolishes this inhibition. Glucose weakly inhibits (K(i)=25-30mm), but trehalose does not inhibit even at 100mm. 6. It is suggested that glucose 6-phosphate is a major regulator of glycogen phosphorylase activity in locust fat-body.
摘要
- 通过差速离心以及在两个pH值下从糖原颗粒解离，对蝗虫脂肪体中的糖原磷酸化酶进行了部分纯化。2. 在pH6.6 - 6.7时获得最佳活性。3. 计算得出糖原和1 - 磷酸葡萄糖的表观K(m)值分别为0.08%和10 - 13mM。4. 5'-AMP在5μM - 1mM范围内具有激活作用。5. 6 - 磷酸葡萄糖是底物1 - 磷酸葡萄糖的竞争性抑制剂（K(i)=1.7mM）。5'-AMP消除这种抑制作用。葡萄糖具有微弱抑制作用（K(i)=25 - 30mM），但海藻糖即使在100mM时也无抑制作用。6. 有人提出，6 - 磷酸葡萄糖是蝗虫脂肪体中糖原磷酸化酶活性的主要调节因子。
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