Kowalewski K, Schier J F, Otto W
Eur Surg Res. 1979;11(1):71-80. doi: 10.1159/000128053.
6 canine livers were perfused extracorporeally with bloodless fluorocarbon emulsion for 6 h. Perfusate was oxygenated in vitro with a mixture of oxygen and carbon dioxide. Biochemical studies were performed on the samples of perfusate taken from the arterial and venous lines of the perfusion circuit, and of the bile. Hepatic tissue was sampled prior to and following 6 h of perfusion for histological and biochemical studies. Changes, if any, found in the concentrations of various components of perfusate and bile, were considered due to the metabolic activity of the liver. Moderate changes of the liver architecture and a decrease of glycogen were found in hepatic tissues studied microscopically. Under the conditions of the present preliminary experiment, canine livers remained functional after 6 h of perfusion.
用无血氟碳乳剂对6只犬肝脏进行体外灌注6小时。灌注液在体外用氧气和二氧化碳的混合气体进行氧合。对从灌注回路的动脉和静脉管路以及胆汁中采集的灌注液样本进行生化研究。在灌注6小时之前和之后采集肝组织样本用于组织学和生化研究。灌注液和胆汁中各种成分浓度的任何变化都被认为是肝脏代谢活动所致。在显微镜下研究的肝组织中发现肝脏结构有中度变化且糖原减少。在本初步实验条件下，犬肝脏在灌注6小时后仍保持功能。
