Kowalewski K, Kolodej A
Eur Surg Res. 1978;10(5):322-8. doi: 10.1159/000128022.
Isolated canine stomachs were perfused for 6 h with fluorocarbon emulsion suspended in a simulated physiological solution of electrolytes with glucose added. Gastric mucosa and extramucosal tissues of perfused stomachs were sampled for biochemical analysis of high energy phosphates, glucose and electrolytes. Comparable samples were also taken from normal canine stomachs, dissected under similar surgical conditions as the stomachs used for perfusion. Gastric tissue ATP, ADP and AMP were reduced but CP was increased in the tissues perfused with fluorocarbon as compared with controls. Gastric tissue water content and sodium were increased but potassium was reduced in a stomach so perfused. The biochemical tests performed were considered as viability tests of organs preserved-perfused in fluorocarbon prior to possible transplantation. Significance of the changes observed is discussed in the light of the current knowledge on energy metabolism.
将分离出的犬胃用悬浮于添加了葡萄糖的模拟生理电解质溶液中的氟碳乳剂灌注6小时。对灌注胃的胃黏膜和黏膜外组织进行取样，以分析高能磷酸盐、葡萄糖和电解质的生化指标。还从正常犬胃中采集了类似样本，这些胃是在与用于灌注的胃相似的手术条件下进行解剖的。与对照组相比，用氟碳灌注的组织中胃组织的三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和一磷酸腺苷（AMP）减少，但磷酸肌酸（CP）增加。如此灌注的胃中，胃组织含水量和钠含量增加，但钾含量减少。所进行的生化测试被视为在可能进行移植之前在氟碳中保存 - 灌注的器官的活力测试。根据当前关于能量代谢的知识讨论了观察到的变化的意义。
