一种评估多分散制剂分子量分布的新通用方法。其在一种肠上皮糖蛋白和两种葡聚糖样品中的应用，以及与一种单分散糖蛋白的比较。
A new general method for the assessment of the molecular-weight distribution of polydisperse preparations. Its application to an intestinal epithelial glycoprotein and two dextran samples, and comparison with a monodisperse glycoprotein.
作者信息
Gibbons R A, Dixon S N, Pocock D H
出版信息
Biochem J. 1973 Dec;135(4):649-55. doi: 10.1042/bj1350649.
A specimen of intestinal glycoprotein isolated from the pig and two samples of dextran, all of which are polydisperse (that is, the preparations may be regarded as consisting of a continuous distribution of molecular weights), have been examined in the ultracentrifuge under meniscus-depletion conditions at equilibrium. They are compared with each other and with a glycoprotein from Cysticercus tenuicollis cyst fluid which is almost monodisperse. The quantity c(-(1/3)) (c=concentration) is plotted against xi (the reduced radius); this plot is linear when the molecular-weight distribution approximates to the ;most probable', i.e. when M(n):M(w):M(z): M((z+1))....... is as 1:2:3:4: etc. The use of this plot, and related procedures, to evaluate qualitatively and semi-quantitatively molecular-weight distribution functions where they can be realistically approximated to Schulz distributions is discussed. The theoretical basis is given in an Appendix.
从猪身上分离出的一种肠糖蛋白标本以及两种葡聚糖样品，所有这些都是多分散的（也就是说，这些制剂可被视为由连续的分子量分布组成），已在平衡状态下弯月面耗尽条件下用超速离心机进行了检测。将它们相互比较，并与来自细颈囊尾蚴囊液的几乎单分散的糖蛋白进行比较。绘制c^(-1/3)（c = 浓度）对ξ（折合半径）的图；当分子量分布近似于“最可几”时，即当M(n):M(w):M(z):M((z + 1)).......为1:2:3:4:等时，该图呈线性。讨论了使用该图及相关程序在分子量分布函数可实际近似为舒尔茨分布的情况下进行定性和半定量评估的问题。附录中给出了理论依据。
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