Bruggeman T M
Gastroenterology. 1975 May;68(5 Pt 1):1204-10.
Because it has not been possible to collect gastric lymph by cannulation of lymph vessels, the methods of renal micropuncture were adapted to collection and analysis of 0.5- to 3-mu samples obtained by puncturing lymph vessels on or immediately adjacent to the ventral surface of the dog's stomach. Samples of gastric lymph collected immediately after completion of surgical exposure of the stomach were subjected to acrylamide gel electrophoresis, and they were found to show all the protein bands exhibited by simultaneously collected samples of plasma. Quantitative analysis of similar samples by cellulose acetate electrophoresis gave the following ratios of protein concentration in gastric lymph to plasma: total protein, 0.51; albumin, 0.68; total globulins, 0.42; alpha1-globulin, 0.49; alpha2-globulin, 0.62; beta-globulin, 0.35; fibrinogen, 0.39; and gamma-globulin, 0.46. When samples were collected over 4 hr the concentrations of all proteins in lymph increased, and an argument is presented that this was not the result of increasing small pore size as the result of injury. When the mucosa was irrigated with solutions of dithiothreitol, a substance causing plasma shedding, the concentrations of proteins in gastric lymph over a 4-hr period were similar to those found in control experiments. Gastric lymph mixed with 100 mN HCl was found to have proteolytic activity.
由于通过淋巴管插管收集胃淋巴液是不可能的，因此采用了肾脏微穿刺方法来收集和分析通过穿刺犬胃腹面或其紧邻处的淋巴管获得的0.5至3微升样本。在完成胃的手术暴露后立即收集的胃淋巴样本进行了丙烯酰胺凝胶电泳，结果发现它们显示出与同时收集的血浆样本相同的所有蛋白带。通过醋酸纤维素电泳对类似样本进行定量分析，得到胃淋巴液与血浆中蛋白质浓度的以下比率：总蛋白，0.51；白蛋白，0.68；总球蛋白，0.42；α1球蛋白，0.49；α2球蛋白，0.62；β球蛋白，0.35；纤维蛋白原，0.39；γ球蛋白，0.46。当在4小时内收集样本时，淋巴液中所有蛋白质的浓度均升高，并且有人认为这不是损伤导致小孔径增加的结果。当用二硫苏糖醇溶液（一种导致血浆渗出的物质）冲洗粘膜时，4小时内胃淋巴液中蛋白质的浓度与对照实验中发现的浓度相似。发现与100 mN HCl混合的胃淋巴液具有蛋白水解活性。
