Roizes G, Pages M, Lecou C, Patillon M, Kovoor A
Gene. 1979 May;6(1):43-50. doi: 10.1016/0378-1119(79)90084-2.
AtuBVI, an endonuclease showing new site-specificity, has been isolated from the tumorigenic strain IIBV7 of Agrobacterium tumefaciens, and is undetectable in the non-tumorigenic sister strain IIBNV6. AtuBVI degrades IIBV7 DNA in vitro and should, therefore, be regarded as being phenotypically cryptic in the bacterial cell; it also shows anomalous behavior under cerain incubation conditions. These properties point to a possible role for this enzyme in the insertion of exogenous Ti-plasmid DNA into plant tissues during tumorigenesis.
AtuBVI是一种具有新位点特异性的核酸内切酶,已从根癌土壤杆菌的致瘤菌株IIBV7中分离出来,而在非致瘤姊妹菌株IIBNV6中未检测到。AtuBVI在体外可降解IIBV7 DNA,因此在细菌细胞中应被视为表型隐性;它在某些孵育条件下也表现出异常行为。这些特性表明该酶在肿瘤发生过程中可能参与将外源Ti质粒DNA插入植物组织。