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一种新的位点特异性内切核酸酶,在根癌土壤杆菌的致瘤菌株中表现出表型隐匿性。

A new site-specific endonuclease showing phenotypical crypticity in a tumorigenic strain of Agrobacterium tumefaciens.

作者信息

Roizes G, Pages M, Lecou C, Patillon M, Kovoor A

出版信息

Gene. 1979 May;6(1):43-50. doi: 10.1016/0378-1119(79)90084-2.

DOI:10.1016/0378-1119(79)90084-2
PMID:478298
Abstract

AtuBVI, an endonuclease showing new site-specificity, has been isolated from the tumorigenic strain IIBV7 of Agrobacterium tumefaciens, and is undetectable in the non-tumorigenic sister strain IIBNV6. AtuBVI degrades IIBV7 DNA in vitro and should, therefore, be regarded as being phenotypically cryptic in the bacterial cell; it also shows anomalous behavior under cerain incubation conditions. These properties point to a possible role for this enzyme in the insertion of exogenous Ti-plasmid DNA into plant tissues during tumorigenesis.

摘要

AtuBVI是一种具有新位点特异性的核酸内切酶,已从根癌土壤杆菌的致瘤菌株IIBV7中分离出来,而在非致瘤姊妹菌株IIBNV6中未检测到。AtuBVI在体外可降解IIBV7 DNA,因此在细菌细胞中应被视为表型隐性;它在某些孵育条件下也表现出异常行为。这些特性表明该酶在肿瘤发生过程中可能参与将外源Ti质粒DNA插入植物组织。

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A new site-specific endonuclease showing phenotypical crypticity in a tumorigenic strain of Agrobacterium tumefaciens.一种新的位点特异性内切核酸酶,在根癌土壤杆菌的致瘤菌株中表现出表型隐匿性。
Gene. 1979 May;6(1):43-50. doi: 10.1016/0378-1119(79)90084-2.
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[Site-specific restriction endonuclease RME211 from Rhizobium meliloti].[来自苜蓿根瘤菌的位点特异性限制性内切酶RME211]
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Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti-plasmid and the plant host.根癌土壤杆菌、Ti 质粒与植物宿主之间的相互作用及 DNA 转移
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Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens.根癌土壤杆菌中由virD编码的边界特异性核酸内切酶在大肠杆菌中对T-DNA进行双链切割并生成单链T-DNA分子。
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Nucleic Acids Res. 1979 Dec 11;7(7):1837-49. doi: 10.1093/nar/7.7.1837.

引用本文的文献

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Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1981 Jan 10;9(1):r75-96. doi: 10.1093/nar/9.1.213-c.
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Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1980 Jan 11;8(1):r63-r80. doi: 10.1093/nar/8.1.197-d.
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Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1985;13 Suppl(Suppl):r165-200. doi: 10.1093/nar/13.suppl.r165.