• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

根癌土壤杆菌的DNA修饰酶：DNA拓扑异构酶、限制性内切酶和独特DNA内切酶对质粒和植物DNA的影响

DNA modifying enzymes of Agrobacterium tumefaciens: effect of DNA topoisomerase, restriction endonuclease, and unique DNA endonuclease on plasmid and plant DNA.

作者信息

LeBon J M, Kado C I, Rosenthal L J, Chirikjian J G

出版信息

Proc Natl Acad Sci U S A. 1978 Sep;75(9):4097-101. doi: 10.1073/pnas.75.9.4097.

DOI:10.1073/pnas.75.9.4097
PMID:212732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC336058/
Abstract

Extracts from Agrobacterium tumefaciens strain ID135 contain three enzymes that have been characterized and partially purified. The first enzyme, a DNA topoisomerase, appeared to relax only negatively twisted DNA. The second enzyme, Atu I, a type II restriction endonuclease, generated the identical DNA digestion pattern as EcoRII when several DNAs were used. The third enzyme, endonuclease A, showed a preference for superhelical DNAs as substrates. When plasmid pCK135DNA, obtained from the virulent strain IDI135 of A. tumefaciens, or plant DNA was exposed to the three enzymes, changes in DNA patterns were observed due to either conformational changes or digestion of the DNAs. These enzymes may function in vivo in the processing and incorporation of bacterial DNA in plant cells.

摘要

根癌土壤杆菌菌株ID135的提取物含有三种已被鉴定并部分纯化的酶。第一种酶是一种DNA拓扑异构酶，似乎只使负超螺旋DNA松弛。第二种酶Atu I是一种II型限制性内切酶，当使用几种DNA时，它产生的DNA消化模式与EcoRII相同。第三种酶核酸内切酶A表现出偏好以超螺旋DNA作为底物。当从根癌土壤杆菌的致病菌株IDI135获得的质粒pCK135DNA或植物DNA暴露于这三种酶时，由于DNA的构象变化或消化，观察到了DNA模式的变化。这些酶可能在体内参与细菌DNA在植物细胞中的加工和整合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/377e88babcd6/pnas00668-0038-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/cab360dfcd8c/pnas00668-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/18a9055fdbd1/pnas00668-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/7d2695cbc52d/pnas00668-0037-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/4a7e2525aa7f/pnas00668-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/377e88babcd6/pnas00668-0038-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/cab360dfcd8c/pnas00668-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/18a9055fdbd1/pnas00668-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/7d2695cbc52d/pnas00668-0037-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/4a7e2525aa7f/pnas00668-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1137/336058/377e88babcd6/pnas00668-0038-b.jpg

相似文献

1
DNA modifying enzymes of Agrobacterium tumefaciens: effect of DNA topoisomerase, restriction endonuclease, and unique DNA endonuclease on plasmid and plant DNA.根癌土壤杆菌的DNA修饰酶：DNA拓扑异构酶、限制性内切酶和独特DNA内切酶对质粒和植物DNA的影响
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4097-101. doi: 10.1073/pnas.75.9.4097.
2
Correlation of enzyme-induced cleavage sites on negatively superhelical DNA between prokaryotic topoisomerase I and S1 nuclease.原核生物拓扑异构酶I与S1核酸酶在负超螺旋DNA上酶诱导切割位点的相关性
Biochim Biophys Acta. 1983 May 20;740(1):108-17. doi: 10.1016/0167-4781(83)90127-6.
3
The virD operon of Agrobacterium tumefaciens Ti plasmid encodes a DNA-relaxing enzyme.根癌土壤杆菌Ti质粒的virD操纵子编码一种DNA松弛酶。
Proc Natl Acad Sci U S A. 1989 May;86(9):3109-13. doi: 10.1073/pnas.86.9.3109.
4
A restriction endonuclease from Agrobacterium tumefaciens.一种来自根癌土壤杆菌的限制性内切酶。
FEBS Lett. 1977 Oct 1;82(1):69-70. doi: 10.1016/0014-5793(77)80887-9.
5
A new site-specific endonuclease showing phenotypical crypticity in a tumorigenic strain of Agrobacterium tumefaciens.一种新的位点特异性内切核酸酶，在根癌土壤杆菌的致瘤菌株中表现出表型隐匿性。
Gene. 1979 May;6(1):43-50. doi: 10.1016/0378-1119(79)90084-2.
6
The reactions of the EcoRi and other restriction endonucleases.EcoRi及其他限制性内切酶的反应
Biochem J. 1979 May 1;179(2):353-65. doi: 10.1042/bj1790353.
7
Restriction endonuclease mapping of a plasmid that confers oncogenicity upon Agrobacterium tumefaciens strain B6-806.对赋予根癌土壤杆菌B6 - 806菌株致癌性的质粒进行限制性内切酶图谱分析。
Plasmid. 1978 Feb;1(2):254-69. doi: 10.1016/0147-619x(78)90043-4.
8
The SalGI restriction endonuclease. Enzyme specificity.SalGI限制性核酸内切酶。酶的特异性。
Biochem J. 1982 Apr 1;203(1):93-8. doi: 10.1042/bj2030093.
9
[Site-specific restriction endonuclease RME211 from Rhizobium meliloti].[来自苜蓿根瘤菌的位点特异性限制性内切酶RME211]
Mol Gen Mikrobiol Virusol. 1991 Mar(3):30-1.
10
Purification, properties and determinations of recognition sequence and cleavage site of restriction endonuclease from "Agrobacterium gelatinovorum" IAM 12617, a marine bacterium (AgeI).从海洋细菌“明胶土壤杆菌”IAM 12617（AgeI）中提取的限制性内切酶的纯化、性质以及识别序列和切割位点的测定
Agric Biol Chem. 1990 Jul;54(7):1797-802.

引用本文的文献

1
Agrobacterium Ti plasmid indoleacetic acid gene is required for crown gall oncogenesis.根癌农杆菌 Ti 质粒吲哚乙酸基因是诱发冠瘿瘤发生所必需的。
Proc Natl Acad Sci U S A. 1982 May;79(9):2812-6. doi: 10.1073/pnas.79.9.2812.
2
Methylation of the T-DNA in Agrobacterium tumefaciens and in several crown gall tumors.根癌土壤杆菌及多种冠瘿瘤中T-DNA的甲基化
Nucleic Acids Res. 1983 Jan 11;11(1):159-74. doi: 10.1093/nar/11.1.159.
3
Mapping the in vivo arrangement of nucleosomes on simian virus 40 chromatin by the photoaddition of radioactive hydroxymethyltrimethylpsoralen.

本文引用的文献

1
A PLANT-TUMOR OF BACTERIAL ORIGIN.一种细菌起源的植物肿瘤。
Science. 1907 Apr 26;25(643):671-3. doi: 10.1126/science.25.643.671.
2
COHESION OF DNA MOLECULES ISOLATED FROM PHAGE LAMBDA.从噬菌体λ中分离出的DNA分子的凝聚作用
Proc Natl Acad Sci U S A. 1963 May;49(5):748-55. doi: 10.1073/pnas.49.5.748.
3
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
通过放射性羟甲基三甲基补骨脂素的光加成作用绘制猿猴病毒40染色质上核小体的体内排列图谱。
J Virol. 1982 Jan;41(1):78-87. doi: 10.1128/JVI.41.1.78-87.1982.
4
Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1982 Mar 11;10(5):r117-44. doi: 10.1093/nar/10.5.1770.
5
Uncoupling of SV40 tsA replicon activation from DNA chain elongation by temperature shifts and aphidicolin arrest.通过温度变化和阿非科林阻滞使SV40 tsA复制子激活与DNA链延伸解偶联。
Nucleic Acids Res. 1982 Jan 22;10(2):763-73. doi: 10.1093/nar/10.2.763.
6
Cloning of bacteriophage fd gene 2 and construction of a plasmid dependent on fd gene 2 protein.噬菌体fd基因2的克隆及依赖fd基因2蛋白的质粒构建。
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5416-20. doi: 10.1073/pnas.78.9.5416.
7
DNA topoisomerase from Agrobacterium tumefaciens: purification and catalytic properties.根癌土壤杆菌的DNA拓扑异构酶：纯化及催化特性
Nucleic Acids Res. 1981 Feb 25;9(4):909-20. doi: 10.1093/nar/9.4.909.
8
Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1981 Jan 10;9(1):r75-96. doi: 10.1093/nar/9.1.213-c.
9
RsaI: a new sequence-specific endonuclease activity from Rhodopseudomonas sphaeroides.RsaI：一种来自球形红假单胞菌的新型序列特异性核酸内切酶活性
J Bacteriol. 1980 May;142(2):380-3. doi: 10.1128/jb.142.2.380-383.1980.
10
Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1980 Jan 11;8(1):r63-r80. doi: 10.1093/nar/8.1.197-d.
J Biol Chem. 1956 Jan;218(1):97-106.
4
Enzymatic breakage and joining of deoxyribonucleic acid. V. End group labeling and analysis of deoxyribonucleic acid containing single straned breaks.脱氧核糖核酸的酶促断裂与连接。V. 末端基团标记及含单链断裂的脱氧核糖核酸分析
J Biol Chem. 1968 Sep 10;243(17):4530-42.
5
The loss of tumor-initiating ability in Agrobacterium tumefaciens by incubation at high temperature.通过高温培养使根癌土壤杆菌丧失肿瘤起始能力。
Experientia. 1971 Feb 15;27(2):229-30. doi: 10.1007/BF02145913.
6
Alkali denaturation of covalently closed circular duplex deoxyribonucleic acid.共价闭合环状双链脱氧核糖核酸的碱变性
J Biol Chem. 1970 May 25;245(10):2704-8.
7
The 3'-terminal nucleotide sequences of T7 DNA.T7 DNA的3'末端核苷酸序列。
J Mol Biol. 1972 May 14;66(2):209-24. doi: 10.1016/0022-2836(72)90474-3.
8
Interaction between DNA and an Escherichia coli protein omega.DNA与大肠杆菌蛋白ω之间的相互作用。
J Mol Biol. 1971 Feb 14;55(3):523-33. doi: 10.1016/0022-2836(71)90334-2.
9
Supercoiled circular DNA in crown-gall inducing Agrobacterium strains.冠瘿诱导农杆菌菌株中的超螺旋环状DNA。
J Mol Biol. 1974 Jun 15;86(1):109-27. doi: 10.1016/s0022-2836(74)80011-2.
10
Recognition sequence of a restriction enzyme.限制酶的识别序列。
Nat New Biol. 1973 Jul 4;244(131):7-10. doi: 10.1038/newbio244007a0.