Hertz E, Hertz L
In Vitro. 1979 Jun;15(6):429-36. doi: 10.1007/BF02618411.
Oxygen uptake was measured in primary cultures of astrocytes from the brain hemispheres of newborn DBA mice by the aid of an oxygen electrode inserted directly into the culture flask, i.e. using the flask, completely filled with MEM medium, as the respirometer chamber. The respiration was initially intense (300 mumol per hr per 100 mg protein) but declined somewhat during the 6 hr of measurement, probably due to a depletion of intermediary metabolites released to the large surplus of medium. The respiratory rates were approximately identical in the presence of a CO2/bicarbonate and a HEPES buffer. Exposure to a high concentration of potassium led to a transient stimulation of the oxygen uptake to almost 100%, a response that was very easily observed using the present method. Since no mechanical damage was inflicted upon the cells, culturing could be continued, if so desired, after the measurement.
借助直接插入培养瓶的氧电极，在新生DBA小鼠脑半球星形胶质细胞的原代培养物中测量氧摄取，即使用完全充满MEM培养基的培养瓶作为呼吸计室。呼吸最初很强烈（每小时每100毫克蛋白质300微摩尔），但在测量的6小时内有所下降，可能是由于释放到大量培养基中的中间代谢产物耗尽。在存在二氧化碳/碳酸氢盐和HEPES缓冲液的情况下，呼吸速率大致相同。暴露于高浓度钾会导致氧摄取短暂刺激至几乎100%，使用本方法很容易观察到这种反应。由于未对细胞造成机械损伤，如果需要，测量后可以继续培养。
