Rehm S R, Gross G N, Hart D A, Pierce A K
Infect Immun. 1979 Jul;25(1):299-303. doi: 10.1128/iai.25.1.299-303.1979.
When two sets of phagocytic cells participate simultaneously in the inflammatory process and bacterial killing, the relative contribution of each cell type is difficult to ascertain. The use of cell-specific antibody will permit selective depletion of one phagocyte population. We describe an experimental model of granulocytopenia which utilizes the immunoglobulin G fraction of an antigranulocyte serum. This material markedly depleted circulating polymorphonuclear leukocytes (PMN); within 2 h after injection of antigranulocyte globulin, PMN counts were at 19% of original levels and remained significantly depressed for 24 h. Granulocyte recruitment was also impaired, with only 5 x 10(3) PMN appearing in the lungs in response to an aerosol of Klebsiella, compared to 4.17 x 10(5) PMN in control animals (P less than 0.01). Most importantly, alveolar macrophages retained normal viability (97% versus 94% for control value, P not significant) normal phagocytic function, and normal bactericidal capacity. Antigranulocyte globulin is thus a valuable tool for the study of bacterial defense mechanisms.
当两组吞噬细胞同时参与炎症过程和细菌杀灭时，每种细胞类型的相对贡献难以确定。使用细胞特异性抗体将允许选择性清除一种吞噬细胞群体。我们描述了一种粒细胞减少症的实验模型，该模型利用抗粒细胞血清的免疫球蛋白G部分。这种物质显著减少了循环中的多形核白细胞（PMN）；注射抗粒细胞球蛋白后2小时内，PMN计数降至原始水平的19%，并在24小时内仍显著降低。粒细胞募集也受到损害，与对照动物中4.17×10⁵个PMN相比，对肺炎克雷伯菌气雾剂产生反应时，肺部仅出现5×10³个PMN（P<0.01）。最重要的是，肺泡巨噬细胞保持正常活力（与对照值相比为97%对94%，P无显著性差异）、正常吞噬功能和正常杀菌能力。因此，抗粒细胞球蛋白是研究细菌防御机制的有价值工具。
