呼吸道黏蛋白的生物合成。犬上皮杯状细胞与黏膜下腺分泌物的比较。
Biosynthesis of respiratory-tract mucins. A comparison of canine epithelial goblet-cell and submucosal-gland secretions.
作者信息
Stahl G H, Ellis D B
出版信息
Biochem J. 1973 Dec;136(4):845-50. doi: 10.1042/bj1360845.
Abstract
- A modified canine tracheal organ culture system was used to investigate differences between mucous secretions of epithelial goblet cells and the submucosal glands. 2. Denuded explants were prepared by removing goblet, ciliated and basal cells from the surface epithelium leaving an intact basement membrane and viable submucosa. 3. Denuded explants actively incorporated radioactive precursors into secreted macromolecules when cultured in medium 199 containing label. 4. Chromatography on Bio-Gel A-150m and electrophoresis on 1% agarose gels indicated that epithelial goblet cell secretions were relatively more sulphated than submucosal glandular secretions. 5. The glandular structures were shown to respond to a parasympathomimetic agent.
摘要
- 采用改良的犬气管器官培养系统来研究上皮杯状细胞和黏膜下腺黏液分泌之间的差异。2. 通过从表面上皮去除杯状细胞、纤毛细胞和基底细胞制备裸露外植体，留下完整的基底膜和存活的黏膜下层。3. 当在含有标记物的199培养基中培养时，裸露外植体将放射性前体积极掺入分泌的大分子中。4. 在Bio-Gel A-150m上进行色谱分析和在1%琼脂糖凝胶上进行电泳表明，上皮杯状细胞分泌物比黏膜下腺分泌物硫酸化程度相对更高。5. 已表明腺结构对拟副交感神经药有反应。
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本文引用的文献
1
INTRACELLULAR MUCOPOLYSACCHARIDES IN THE NORMAL HUMAN BRONCHIAL TREE.正常人支气管树中的细胞内粘多糖
Q J Exp Physiol Cogn Med Sci. 1964 Jan;49:85-94. doi: 10.1113/expphysiol.1964.sp001709.
2
A cytological basis for the biochemical study of bronchial epithelium.支气管上皮生化研究的细胞学基础。
J Histochem Cytochem. 1958 Mar;6(2):105-11. doi: 10.1177/6.2.105.
3
Histochemical types of acidic glycoprotein produced by mucous cells of the tracheobronchial glands in man.人类气管支气管腺黏液细胞产生的酸性糖蛋白的组织化学类型。
J Pathol. 1969 Aug;98(4):213-29. doi: 10.1002/path.1710980402.
4
Gel electrophoresis of mucous glycoproteins. II. Effect of physical deaggregation and disulfide-bond cleavage.黏液糖蛋白的凝胶电泳。II. 物理解聚和二硫键断裂的影响。
Biochemistry. 1971 Aug 3;10(16):3110-3. doi: 10.1021/bi00792a020.
5
[Isolation of bronchial mucins secreted in cystic fibrosis].[囊性纤维化中分泌的支气管粘蛋白的分离]
Clin Chim Acta. 1972 Feb;36(2):315-28. doi: 10.1016/0009-8981(72)90005-8.
6
Secretory activity of the human bronchial mucous glands in vitro.
Exp Mol Pathol. 1972 Jun;16(3):362-81. doi: 10.1016/0014-4800(72)90011-1.
7
[Biochemical data on the bronchial mucus of sputum].
Prensa Med Argent. 1966;53(1):426-35.
8
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