Siew S
Isr J Med Sci. 1979 Aug;15(8):698-710.
This report deals with the investigation of renal crystallosis in the autopsy tissue of a fatal human case of ethylene glycol poisoning. A previously healthy 22-year-old man consumed about one liter of Prestone antifreeze, which contains 95% by weight of ethylene glycol. He died in coma 48 h later with clinical evidence of metabolic acidosis and renal shutdown. Postmortem histological examination showed abundant crystallosis of the renal tubules. Polarization microscopy demonstrated brilliant birefringence of the crystals. In addition to the tubular crystallosis, scanning electron microscopy showed crystalline particles within the glomeruli as well. Energy dispersive X-ray analysis of the crystals gave a strong calcium signal. The crystals were demonstrated by means of conventional and high-voltage electron microscopy. Electron diffraction gave several hexagonal patterns of calcium carbonate-calcite and a tetragonal pattern suggestive of calcium oxalate monohydrate. X-ray diffraction of the crystals separated from the kidney showed the presence of calcium carbonate and calcium oxalate monohydrate as the major constituents.
本报告涉及对一例致命的乙二醇中毒人类尸检组织中肾结晶症的调查。一名此前健康的22岁男性饮用了约一升含有95%（重量）乙二醇的普雷斯通防冻液。48小时后，他死于昏迷，有代谢性酸中毒和肾衰竭的临床证据。尸检组织学检查显示肾小管有大量结晶症。偏光显微镜显示晶体有明显的双折射。除了肾小管结晶症外，扫描电子显微镜还显示肾小球内也有结晶颗粒。对晶体进行能量色散X射线分析得到强烈的钙信号。通过常规和高压电子显微镜观察到了这些晶体。电子衍射给出了几种碳酸钙-方解石的六边形图案和一种提示一水合草酸钙的四方图案。从肾脏分离出的晶体的X射线衍射显示，主要成分是碳酸钙和一水合草酸钙。
