一种针对霉菌毒素桔青霉素的间接酶免疫测定法。
An indirect enzyme immunoassay for the mycotoxin citrinin.
作者信息
Abramson D, Usleber E, Märtlbauer E
机构信息
Agriculture and Agri-Food Canada, Winnipeg Research Center, Manitoba.
出版信息
Appl Environ Microbiol. 1995 May;61(5):2007-9. doi: 10.1128/aem.61.5.2007-2009.1995.
Abstract
An indirect competitive enzyme immunoassay using rabbit antisera could detect citrinin in buffer solutions at 1 to 13 ng/ml (0.05 to 0.65 ng per assay). Cross-reactivity with austdiol, alternariol, ochratoxin A, and deoxynivalenol was < 0.1% relative to citrinin. Recovery of citrinin added to wheat flour at 200 to 2,000 ng/g was 89 to 104%, with a coefficient of variation of 6.9 to 13%.
摘要
使用兔抗血清的间接竞争酶免疫分析法能够检测缓冲溶液中浓度为1至13纳克/毫升（每次检测0.05至0.65纳克）的桔青霉素。与奥司他二醇、交链孢酚、赭曲霉毒素A和脱氧雪腐镰刀菌烯醇的交叉反应率相对于桔青霉素小于0.1%。添加到小麦粉中浓度为200至2000纳克/克的桔青霉素回收率为89%至104%，变异系数为6.9%至13%。
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