Colliflower E J, Thrasher J J
J Assoc Off Anal Chem. 1979 May;62(3):597-9.
The official methods for extracting light filth from rubbed sage, 44.D08--44.D10, specifies a hot isopropanol pretreatment and flotation from cooled dilute isopropanol with mineral oil to extract light filth. The method gives good recoveries, but occasionally excessive interfering plant material is extracted along with the filth elements. A new method has been developed in which chloroform is used for pretreatment instead of isopropanol, and Tween 80-EDTA is added twice rather than once. This method has given consistently cleaner filter papers and better recoveries for rodent hairs, 85 vs. 79%, and for elytral squares, 95 vs. 84%. The new method has been adopted as official first action.
从磨碎的鼠尾草中提取轻质污垢的官方方法44.D08 - 44.D10规定了用热异丙醇进行预处理，并在冷却的稀异丙醇中加入矿物油进行浮选以提取轻质污垢。该方法回收率良好，但偶尔会在提取污垢成分的同时提取过多干扰性植物物质。已开发出一种新方法，其中用氯仿代替异丙醇进行预处理，并将吐温80 - 乙二胺四乙酸添加两次而非一次。该方法得到的滤纸始终更干净，对啮齿动物毛发的回收率更高，分别为85%对79%，对鞘翅方块的回收率分别为95%对84%。新方法已被采用为官方首次行动方法。
