Obinata T, Shimada Y, Matsuda R
J Cell Biol. 1979 Apr;81(1):59-66. doi: 10.1083/jcb.81.1.59.
The fine structurel distribution of troponin on thin filaments in developing myofibrils was investigated by the use of immunoelectron microscopy. Embryonic chick skeletal muscle cells grown in vitro were treated with antibodies against each of the troponin components (troponin T, I, and C) from adult chicken muscles. Each antibody was distributed along the thin filaments with a period of 38 nm. It is concluded that these newly synthesized regulatory proteins are assembled at their characteristic position from the initial phases of myofibrillogenesis.
利用免疫电子显微镜研究了肌钙蛋白在发育中的肌原纤维细肌丝上的精细结构分布。体外培养的胚胎鸡骨骼肌细胞用针对成年鸡肌肉中每种肌钙蛋白成分（肌钙蛋白T、I和C）的抗体进行处理。每种抗体沿细肌丝以38纳米的周期分布。得出的结论是，这些新合成的调节蛋白从肌原纤维形成的初始阶段就在其特征位置组装。
