Shimada Y, Fischman D A, Moscona A A
J Cell Biol. 1967 Nov;35(2):445-53. doi: 10.1083/jcb.35.2.445.
Dissociated myoblasts from 12-day chick embryos were cultured in monolayer, and the differentiation of skeletal muscle cells was studied by electron microscopy. The results have revealed a striking ultrastructural similarity between the in vivo and the in vitro developing muscle, particularly with respect to the myofibrils and sarcoplasmic reticulum. This study demonstrates that all the characteristic organelles of mature skeletal muscle can develop in vitro in the absence of nerves.
从12日龄鸡胚中分离出的成肌细胞进行单层培养,并用电子显微镜研究骨骼肌细胞的分化。结果显示,体内和体外发育的肌肉在超微结构上有显著相似性,尤其是在肌原纤维和肌浆网方面。这项研究表明,成熟骨骼肌的所有特征性细胞器在没有神经的情况下也能在体外发育。