Nelson T E, Bee D E
J Clin Invest. 1979 Oct;64(4):895-901. doi: 10.1172/JCI109555.
The effects of varying temperatures from 25 degrees to 37 degrees C on calcium binding characteristics of sarcoplasmic reticulum from malignant hyperthermia-susceptible (MHS) and control pig muscle were examined. Two groups of MHS pigs were included: those with high susceptibility to malignant hyperthermia (MHS group) and a cross-bred, less susceptible group (MHX). At 25 degrees C, calcium binding was lower for MHS than for controls and MHX. As temperature was increased by 2 degrees C jumps, calcium binding decreased in all sarcoplasmic reticulum fractions. At 35 degrees C a sharp decrease in calcium binding occurred in the MHS and MHX fractions. The sharp decrease in calcium binding at 35 degrees C differentiated the MHS and MHX fractions from controls. The initial velocity (Vi) for calcium binding was lower in MHS fractions between 25 degrees and 35 degrees C when compared with MHX and controls. All fractions had increased Vi values as temperature increased from 25 degrees to 35 degrees C. From 35 degrees to 39 degrees Vi for controls increased markedly. In contrast, Vi for the MHX fraction decreased as temperature exceeded 35 degrees C. These temperature effects on calcium binding characteristics of sarcoplasmic reticulum from MHS and MHX muscle may be indicative of a membrane transition that impairs calcium binding.
研究了25摄氏度至37摄氏度不同温度对恶性高热易感（MHS）猪和对照猪肌肉肌浆网钙结合特性的影响。纳入了两组MHS猪：对恶性高热高度易感的猪（MHS组）和杂交的、较不易感的猪（MHX组）。在25摄氏度时，MHS组的钙结合能力低于对照组和MHX组。随着温度以2摄氏度的幅度升高，所有肌浆网组分中的钙结合能力均下降。在35摄氏度时，MHS组和MHX组的钙结合能力急剧下降。35摄氏度时钙结合能力的急剧下降使MHS组和MHX组与对照组区分开来。与MHX组和对照组相比，25摄氏度至35摄氏度之间MHS组钙结合的初始速度（Vi）较低。随着温度从25摄氏度升高到35摄氏度，所有组分的Vi值均增加。从35摄氏度到39摄氏度，对照组的Vi显著增加。相比之下，当温度超过35摄氏度时，MHX组的Vi下降。这些温度对MHS组和MHX组肌肉肌浆网钙结合特性的影响可能表明存在损害钙结合的膜转变。
