Erickson J S, Paucker K
J Gen Virol. 1979 Jun;43(3):521-9. doi: 10.1099/0022-1317-43-3-521.
Interferons were stimulated in mouse L cells by Newcastle disease virus (NDV) or by polyriboinosinic-polyribocytidylic acid poly(rI).poly(rC). These were fractionated by sequential affinity chromatography on bovine plasma albumin (BPA)-Sepharose and on omega-carboxypentyl (CH)-Sepharose. Based on their interaction with CH-Sepharose, interferon induced by NDV was resolved into three major bands of activity (L/NDV-1,2,3) and poly(rI).poly(rC)-interferon into two (L/rI:rC-1,2). These interferon components were purified to a specific activity of 3 X 10(7) to 4 X 10(7) units/mg protein by antibody affinity chromatography and examined by electrophoresis in SDS-polyacrylamide gels. A total of five molecular species was thus identified for NDV-induced interferon and three for poly(rI).poly(rC) induced interferon, as summarized in Table 1. We conclude from our observations that mouse interferons can be produced by L cells in multiple forms with specific physiochemical properties and in proportions determined by the type of agent employed for induction.
新城疫病毒（NDV）或聚肌苷酸 - 聚胞苷酸[poly(rI).poly(rC)]可刺激小鼠L细胞产生干扰素。这些干扰素先后通过在牛血浆白蛋白（BPA）-琼脂糖凝胶和ω-羧基戊基（CH）-琼脂糖凝胶上进行亲和层析进行分离。根据它们与CH - 琼脂糖凝胶的相互作用，NDV诱导产生的干扰素被分离为三个主要活性带（L/NDV - 1、2、3），而poly(rI).poly(rC)诱导产生的干扰素被分离为两个活性带（L/rI:rC - 1、2）。通过抗体亲和层析将这些干扰素组分纯化至比活性为3×10⁷至4×10⁷单位/毫克蛋白质，并在SDS - 聚丙烯酰胺凝胶中进行电泳分析。表1总结了NDV诱导产生的干扰素共鉴定出五种分子类型，poly(rI).poly(rC)诱导产生的干扰素共鉴定出三种分子类型。从我们的观察结果得出结论，小鼠干扰素可以由L细胞以多种具有特定理化性质的形式产生，其比例由用于诱导的试剂类型决定。
