Kimura K, Endou H, Sakai F
J Toxicol Sci. 1979 Feb;4(1):1-10. doi: 10.2131/jts.4.1.
The suppressive effect of dipyridamole on the proteinuria of aminonucleoside nephrosis and protamine-induced proteinuria, was investigated. Male Wistar rats were given puromycin aminonucleoside (80 mg/kg s.c.) or protamine sulfate (20 mg/kg i.v.), and the urine was collected in metabolic cages. The content of proteins in the urine was determined by using a continuous gradient microgel electrophoresis procedure. Dipyridamole (20 mg/kg p.o.) suppressed the excretion of albumin and proteins larger than albumin (HMP) in aminonucleoside nephrosis. But the excertion of proteins smaller than albumin (LMP) was not affected by dipyridamole. Dipyridamole also suppressed the excertion of HMP in protamine-induced proteinuria, though the excretion of albumin and LMP was not affected. Puromycin aminonucleoside and protamine sulfate were known to cause renal glomerular epithelial changes referred to as "fusion" of foot processes. Since dipyridamole was effective in suppressing the both types of proteinuria, this drug was considered to improve the damaged renal glomerular barrier for plasma proteins.
研究了双嘧达莫对氨基核苷肾病蛋白尿和鱼精蛋白诱导的蛋白尿的抑制作用。给雄性Wistar大鼠注射嘌呤霉素氨基核苷（80mg/kg，皮下注射）或硫酸鱼精蛋白（20mg/kg，静脉注射），并在代谢笼中收集尿液。采用连续梯度微凝胶电泳法测定尿中蛋白质含量。双嘧达莫（20mg/kg，口服）可抑制氨基核苷肾病中白蛋白及大于白蛋白的蛋白质（HMP）的排泄。但小于白蛋白的蛋白质（LMP）的排泄不受双嘧达莫影响。双嘧达莫也可抑制鱼精蛋白诱导的蛋白尿中HMP的排泄，尽管白蛋白和LMP的排泄不受影响。已知嘌呤霉素氨基核苷和硫酸鱼精蛋白可引起肾小球上皮细胞变化，称为足突“融合”。由于双嘧达莫对两种类型的蛋白尿均有抑制作用，因此认为该药可改善受损的肾小球血浆蛋白屏障。
