Nishiyama S, Kurebe M
J Toxicol Sci. 1979 Feb;4(1):19-29. doi: 10.2131/jts.4.19.
When liver fragments from eleven-day chick embryos were maintained on Eagle's minimal essential medium by the established method of organ culture, they developed ultrastructural features similar to liver cells in vivo, except that they had small amounts of smooth endoplasmic reticulum and little glycogen. The cultured liver cells synthesized DNA, RNA and protein. The addition of aflatoxin B1 to the medium inhibited the synthesis of nucleic acid. Aflatoxin B1 also produced the segregation of granular and fibrillar components in nucleoli and the disarrangement of ribosomes attached to endoplasmic reticulum. Since these results were consistent with the known effects of the toxin in animals, we concluded that organ culture of chick embryo liver could be a useful technique for other studies.
当采用既定的器官培养方法，将11日龄鸡胚的肝组织块在伊格尔氏基本培养基中培养时，它们呈现出与体内肝细胞相似的超微结构特征，只是它们的滑面内质网含量较少且糖原含量很少。培养的肝细胞能合成DNA、RNA和蛋白质。向培养基中添加黄曲霉毒素B1会抑制核酸的合成。黄曲霉毒素B1还会导致核仁中颗粒成分和纤维成分分离，以及附着在内质网上的核糖体排列紊乱。由于这些结果与该毒素在动物体内已知的作用相符，我们得出结论，鸡胚肝的器官培养可能是用于其他研究的一种有用技术。
