Khristov D, Atanasov N, Obreshkova A
Eksp Med Morfol. 1973;12(3):146-50.
The authors examined the activity of acid proteinases in extracts, obtained by 0,05 M KCl from thigh muscles of 12-and 18-day hen embryos and of a 3-year hen and from muscle sarcoma 12--14 days after its transplantation on thigh muscles of a hen. The activity of the fractions obtained from extracts by gel chromatography on sefadex G-200, was also determined. The highiest activity per unit of protein was established in extracts of embryonal muscles, but the lowest -in cultures of hens. Sarcoma took medean position. Seven fraction of acid proteinases were found in the muscles and in the sarcoma. In most cases sarcoma took mean position among embryonal muscles and muscles of hens in percentage ratios of these fractions. The highiest mollecular (the earliest flowing) acid proteinase fractions in the extract of hen muscles had insignificant activity, but in extracts of sarcoma they had almost 20% of the total activity.
作者检测了从12日龄和18日龄鸡胚大腿肌肉、3岁母鸡大腿肌肉以及移植到母鸡大腿肌肉12 - 14天后的肌肉肉瘤中，用0.05M氯化钾提取的提取物中酸性蛋白酶的活性。还测定了通过葡聚糖G - 200凝胶色谱从提取物中获得的各组分的活性。每单位蛋白质的最高活性在胚胎肌肉提取物中测得，而最低活性在母鸡培养物中。肉瘤处于中间位置。在肌肉和肉瘤中发现了七种酸性蛋白酶组分。在大多数情况下，肉瘤在这些组分的百分比比例方面处于胚胎肌肉和母鸡肌肉之间的中间位置。母鸡肌肉提取物中分子量最大（最早流出）的酸性蛋白酶组分活性不显著，但在肉瘤提取物中它们几乎占总活性的20%。
