Dal Canton A, Corradi A, Stanziale R, Maruccio G, Migone L
Kidney Int. 1979 May;15(5):457-62. doi: 10.1038/ki.1979.61.
Glomerular hemodynamics were studied, by micropuncture, in Munich-Wistar rats submitted to 24-hour unilateral ureteral ligation (UUL). Glomerular capillary pressure (PG), intratubular pressure (PT) and pressure in the first-order peritubular capillaries (EAP) were measured with a servonulling device. Single nephron filtration fraction (SNIFF) was calculated fmom arterial and peritubular blood protein concentration. SNGFR was both measured by conventional micropuncture techniques and calculated from efferent arteriole blood flow (EABF) and SNFF. Afferent arteriole blood flow (AABF) and resistance of afferent (Ra) and efferent (Re) arterioles were calculated. Measurements were repeated 1 to 2 hours after the release of the ureter. Sham-operated rats were used as control. UUL caused a marked increase in Ra (from 4.9 +/- [SD] 2.4 to 12.7 +/- 5.1 dynes/sec/cm-5). The fall in SNGFR (from 111.9 +/- [SD] 23.9 to 34.4 +/- 23.1 nl/min/kg body wt) was secondary to a decrease in both PG and AABF. A further increase in Ra (16.0 +/- 6.7 dynes.sec.cm-5) occurred after releasing the ureter. SNGFR, however, was unaltered (33.7 +/- 16.6 nl/min/kg body wt) since PG decreased parallel to PT, but AABF did not significantly change. Conclusion. Ureteral obstruction determines, in 24 hours, a marked cortical ischemia that is not promptly reversed by ureteral release.
采用微穿刺技术研究了慕尼黑-威斯塔大鼠在单侧输尿管结扎24小时（UUL）后的肾小球血流动力学。用伺服零位装置测量肾小球毛细血管压力（PG）、肾小管内压力（PT）和一级肾小管周围毛细血管压力（EAP）。根据动脉血和肾小管周围血蛋白浓度计算单肾单位滤过分数（SNIFF）。SNGFR既通过传统微穿刺技术测量，也根据出球小动脉血流量（EABF）和SNFF计算得出。计算入球小动脉血流量（AABF）以及入球（Ra）和出球（Re）小动脉的阻力。在输尿管松解1至2小时后重复测量。假手术大鼠用作对照。UUL导致Ra显著增加（从4.9±[标准差]2.4增加到12.7±5.1达因/秒/厘米⁻⁵）。SNGFR下降（从111.9±[标准差]23.9降至34.4±23.1纳升/分钟/千克体重）继发于PG和AABF两者的降低。输尿管松解后Ra进一步增加（16.0±6.7达因·秒·厘米⁻⁵）。然而，由于PG与PT平行下降，但AABF没有显著变化，SNGFR未改变（33.7±16.6纳升/分钟/千克体重）。结论：输尿管梗阻在24小时内导致明显的皮质缺血，输尿管松解后不能迅速逆转。
