Gromova L A, Pereverzev N A, Karavaĭko G I
Mikrobiologiia. 1979 Jul-Aug;48(4):689-92.
Spheroplasts were prepared, under the action of lysozyme (200 and 400 mcg/ml) from the cells of Thiobacillus ferrooxidans grown on the Waksman medium with elementary sulfur after their treatment with 0.5M NaCl solutions and exact exposure to sucrose (0.5M). Intracellular structures were revealed on the spheroplasts which could not be detected on intact cells.
在0.5M NaCl溶液处理并精确暴露于蔗糖(0.5M)后,从在含有单质硫的瓦克斯曼培养基上生长的氧化亚铁硫杆菌细胞中,在溶菌酶(200和400 mcg/ml)作用下制备原生质球。在原生质球上发现了完整细胞上无法检测到的细胞内结构。