用甲磺酸甲酯、氮芥或紫外线处理的哺乳动物细胞的恢复。II. S期开始前DNA修复的重要性。
The recovery of mammalian cells treated with methyl methanesolfonate, nitrogen mustard or UV light. II. The importance of DNA repair prior to the initiation of S phase.
作者信息
Clarkson J M, Mitchell D L
出版信息
Mutat Res. 1979 Jul;61(2):343-51. doi: 10.1016/0027-5107(79)90139-8.
CHO cells were synchronized 2 G1 phase and treated with UV light or HN2. These treatments resulted in a dose-dependent reduction in the rate of DNA replication and cell survival. Holding UV-irradiated cells in G1 phase (in HU medium) for an additional 10 h prior to their release into S phase did not assist recovery as measured by either of these criteria. The survival of cells treated with HN2 was also not enhanced by this recovery period. However, following 2 X 10(-5) M HN2 the rate of DNA replication increased from 30% to 70% of the control level when the period in HU medium was extended to 14 h. The induction of cross-links following HN2 treatment of asynchronous cells was shown to be dose dependent. Subsequent incubation in fresh medium resulted in complete recovery within 20 h at concentrations of HN2 up to 10(-5) M, and at 2 X 10(-5) M HN2, 75% of the cross-links were removed at 14 h post treatment.
将中国仓鼠卵巢细胞同步至G1期，并用紫外线或氮芥（HN2）处理。这些处理导致DNA复制速率和细胞存活率呈剂量依赖性降低。在紫外线照射的细胞进入S期之前，将其在G1期（在羟基脲（HU）培养基中）再保持10小时，并不能促进其恢复，这可通过上述任何一项标准来衡量。这段恢复期也未提高经HN2处理的细胞的存活率。然而，在用2×10⁻⁵ M HN2处理后，当在HU培养基中的时间延长至14小时时，DNA复制速率从对照水平的30%增加到70%。结果表明，对非同步细胞进行HN2处理后交联的诱导是剂量依赖性的。在新鲜培养基中随后孵育，在HN2浓度高达10⁻⁵ M时，20小时内可完全恢复，在2×10⁻⁵ M HN2处理后14小时，75%的交联被去除。
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