来自人淋巴细胞连续培养物（Daudi细胞系）的免疫球蛋白的K链具有异常的分子大小。
The K-chains of the immunoglobulin from a continuous culture of human lymphocytes (Daudi) have an unusual molecular size.
作者信息
Kennel S J
出版信息
J Exp Med. 1974 Apr 1;139(4):1031-6. doi: 10.1084/jem.139.4.1031.
Abstract
Membrane-associated immunoglobulin (M-Ig) was isolated from Daudi cells which had been radioiodinated using lactoperoxidase. This M-Ig was found to have a molecular size on SDS-PAGE of 330,000 daltons. The component protein chains (micro and K) have molecular sizes of 75,000 and 33,000 daltons, respectively. Further studies showed that [(3)H]galactose can be incorporated into K-chains, but that extra protein and not carbohydrate was responsible for the increased molecular size of this molecule.
摘要
膜相关免疫球蛋白（M-Ig）是从用乳过氧化物酶进行放射性碘化的Daudi细胞中分离出来的。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上发现这种M-Ig的分子大小为330,000道尔顿。组成蛋白链（μ链和κ链）的分子大小分别为75,000和33,000道尔顿。进一步的研究表明，[³H]半乳糖可以掺入κ链中，但该分子分子大小增加的原因是额外的蛋白质而非碳水化合物。
相似文献
1
The K-chains of the immunoglobulin from a continuous culture of human lymphocytes (Daudi) have an unusual molecular size.来自人淋巴细胞连续培养物（Daudi细胞系）的免疫球蛋白的K链具有异常的分子大小。
J Exp Med. 1974 Apr 1;139(4):1031-6. doi: 10.1084/jem.139.4.1031.
2
Immunoglobulin of T lymphoma cells is an integral membrane protein.T淋巴瘤细胞的免疫球蛋白是一种整合膜蛋白。
Nature. 1974 Dec 13;252(5484):602-4. doi: 10.1038/252602a0.
3
Cell-surface immunoglobulin human thymus cells and its biosynthesis in vitro.细胞表面免疫球蛋白与人胸腺细胞及其体外生物合成
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3716-20. doi: 10.1073/pnas.70.12.3716.
4
Cell surface immunoglobulin. 3. Isolation and characterization of immunoglobulin from nonsecretory human lymphoid cells.细胞表面免疫球蛋白。3. 从非分泌型人淋巴细胞中分离和鉴定免疫球蛋白。
J Exp Med. 1972 Jun 1;135(6):1392-405. doi: 10.1084/jem.135.6.1392.
5
Cell surface immunoglobulin. IX. A new method for the study of synthesis, intracellular transport, and exteriorization in murine splenocytes.细胞表面免疫球蛋白。IX。一种研究小鼠脾细胞中合成、细胞内运输和外化的新方法。
J Exp Med. 1974 Jun 1;139(6):1599-620. doi: 10.1084/jem.139.6.1599.
6
Surface immunoglobulin of a mouse T-cell lymphoma.一种小鼠T细胞淋巴瘤的表面免疫球蛋白。
Immunology. 1974 Nov;27(5):855-61.
7
Properties of an oncornavirus glycoprotein: evidence for its presence on the surface of virions and infected cells.一种肿瘤病毒糖蛋白的特性：关于其存在于病毒粒子和受感染细胞表面的证据。
Virology. 1973 Oct;55(2):464-75. doi: 10.1016/0042-6822(73)90188-8.
8
Immunoglobulin synthesis and secretion. VI. Synthesis and intracellular transport of immunoglobulin in nonsecretory lymphoma cells.免疫球蛋白的合成与分泌。VI. 非分泌性淋巴瘤细胞中免疫球蛋白的合成及细胞内转运
J Exp Med. 1971 Apr 1;133(4):901-20. doi: 10.1084/jem.133.4.901.
9
Synthesis of surface immunoglobulin by lymphocyte leukemia cells in vitro.淋巴细胞白血病细胞在体外合成表面免疫球蛋白。
Eur J Immunol. 1973 Jan;3(1):16-20. doi: 10.1002/eji.1830030105.
10
Solubilization of IgM-kappa specific surface material from Burkitt lymphoma cell lines.从伯基特淋巴瘤细胞系中溶解IgM-κ特异性表面物质。
Immunology. 1971 May;20(5):667-79.
本文引用的文献
1
Immunoglobulin synthesis and secretion. VI. Synthesis and intracellular transport of immunoglobulin in nonsecretory lymphoma cells.免疫球蛋白的合成与分泌。VI. 非分泌性淋巴瘤细胞中免疫球蛋白的合成及细胞内转运
J Exp Med. 1971 Apr 1;133(4):901-20. doi: 10.1084/jem.133.4.901.
2
Cell surface immunoglobulin. 3. Isolation and characterization of immunoglobulin from nonsecretory human lymphoid cells.细胞表面免疫球蛋白。3. 从非分泌型人淋巴细胞中分离和鉴定免疫球蛋白。
J Exp Med. 1972 Jun 1;135(6):1392-405. doi: 10.1084/jem.135.6.1392.
3
4
Complement receptors on cell membranes. I. Evidence for two complement receptors.细胞膜上的补体受体。I. 两种补体受体的证据。
J Immunol. 1973 Nov;111(5):1444-9.
5
Isolation and characterization of plasma membrane associated immunoglobulin from cultured human diploid lymphocytes.从培养的人二倍体细胞淋巴细胞中分离和鉴定与质膜相关的免疫球蛋白
J Mol Biol. 1973 Jun 5;76(4):485-502. doi: 10.1016/0022-2836(73)90487-7.
6
Absence of detectable IgM in enzymatically or biosynthetically labeled thymus-derived lymphocytes.在酶标或生物合成标记的胸腺来源淋巴细胞中未检测到可检测的IgM。
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2879-83. doi: 10.1073/pnas.70.10.2879.
7
A possible precursor of immunoglobulin light chains.免疫球蛋白轻链的一种可能前体。
Nat New Biol. 1972 Sep 27;239(91):117-20. doi: 10.1038/newbio239117a0.
8
Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis.人红细胞膜糖蛋白：通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定的分子量的重新评估。
Biochem Biophys Res Commun. 1971 Jul 16;44(2):390-5. doi: 10.1016/0006-291x(71)90612-7.
9
Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines.体内及衍生培养系中伯基特淋巴瘤细胞上的表面免疫球蛋白M-κ特异性
Cancer Res. 1968 Jul;28(7):1300-10.
10
Cell surface immunoglobulin. II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes.细胞表面免疫球蛋白。II. 从小鼠脾淋巴细胞中分离和鉴定免疫球蛋白。
J Exp Med. 1971 Jul 1;134(1):242-64. doi: 10.1084/jem.134.1.242.
Zotero 插件