细胞表面免疫球蛋白与人胸腺细胞及其体外生物合成
Cell-surface immunoglobulin human thymus cells and its biosynthesis in vitro.
作者信息
Moroz C, Hahn Y
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3716-20. doi: 10.1073/pnas.70.12.3716.
Abstract
Human thymus cells synthesize immunoglobulin in short-term culture, the nascent immunoglobulin appearing in both cytoplasmic and membrane fractions. Surface immunoglobulin was demonstrated by lactoperoxidase radioiodination of the cells. The demonstration of intracellular and surface immunoglobulin required procedures that minimize proteolytic degradation. Noncovalently linked, monomeric mu chains and light chains were found in the cytoplasm and on the surface of the cells by means of acrylamide gel electrophoresis and by specific immunoprecipitation of the isolated chains.
摘要
人胸腺细胞在短期培养中合成免疫球蛋白，新生免疫球蛋白出现在细胞质和膜组分中。通过细胞的乳过氧化物酶放射性碘化证明了表面免疫球蛋白的存在。细胞内和表面免疫球蛋白的证明需要尽量减少蛋白水解降解的程序。通过丙烯酰胺凝胶电泳和分离链的特异性免疫沉淀，在细胞的细胞质和表面发现了非共价连接的单体μ链和轻链。
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