Zabezensky F, Northey W T
Infect Immun. 1974 Feb;9(2):416-8. doi: 10.1128/iai.9.2.416-418.1974.
Guinea pigs were immunized by intramuscular injection of arthrospores from the M11 strain of Coccidioides immitis, and the peritoneal exudate cells were harvested 4 to 6 weeks later. After incubation with various concentrations of coccidioidin in tissue culture, the area of all migration was measured. Results of this study indicate that at a critical level, a variance of 0.1 mug of antigen per ml, determined the difference between approximately 88% migration and 5% migration as compared with control cells incubated in the absence of antigen. The concentration of antigen (ASU-9 stock coccidioidin concentrate) required to produce essentially complete inhibition of migration was determined to be 12.5 mug/ml.
豚鼠通过肌肉注射粗球孢子菌M11菌株的关节孢子进行免疫，4至6周后收集腹腔渗出细胞。在组织培养中与不同浓度的球孢子菌素孵育后，测量所有迁移区域。本研究结果表明，在一个关键水平上，每毫升0.1微克抗原的差异决定了与在无抗原情况下孵育的对照细胞相比，迁移率约为88%和5%之间的差异。产生基本完全抑制迁移所需的抗原浓度（ASU-9储备球孢子菌素浓缩物）被确定为12.5微克/毫升。
