Germanò D, Abbate C, Granata A
Minerva Med. 1979 Sep 15;70(38):2615-9.
The antitoxic action of 1-ascorbic acid and alpha-mercaptopropionyl glycine was examined via the formation of Ehrlich-Heinz bodies in human venous red cells following in vitro intoxication with 100 mg/ml acetylphenylhydrazine. Marked synergism was noted, especially when small amounts of glucose were added. Both substances displayed a marked affinity for red cells and penetrated them on contact without an incubation period. Optimum amounts for maximum synergy were alpha-mercaptopropionyl glycine 0.01-0.02, 1-ascorbic acid 0.01-0.02 ml and glucose 40 mg per ml of blood. At this dose, appearance of Ehrlich bodies was appreciably retarded. In addition, changes in the size and quality of such bodies pointed to enhanced cell resistance to the poison used.
通过在体外用人静脉红细胞与100mg/ml乙酰苯肼一起孵育后形成埃利希 - 海因茨小体，来检测1-抗坏血酸和α-巯基丙酰甘氨酸的抗毒作用。观察到显著的协同作用，尤其是添加少量葡萄糖时。这两种物质对红细胞均表现出显著的亲和力，接触后无需孵育期即可穿透红细胞。实现最大协同作用的最佳用量为：α-巯基丙酰甘氨酸0.01 - 0.02、1-抗坏血酸0.01 - 0.02ml以及每毫升血液中葡萄糖40mg。在此剂量下，埃利希小体的出现明显延迟。此外，此类小体在大小和性质上的变化表明细胞对所用毒物的抗性增强。
