Lachant N A, Tanaka K R
Am J Med Sci. 1986 Jul;292(1):3-10. doi: 10.1097/00000441-198607000-00001.
The authors examined the ability of antioxidants to prevent in vitro oxidant damage to the sickle red blood cell (RBC). One millimolar ascorbic acid and alpha-mercaptopropionylglycine significantly (p less than 0.005) protected against RBC Heinz body formation during incubation with acetylphenylhydrazine, while cysteine, cysteamine, and methionine did not. The effect of ascorbic acid was concentration dependent with concentrations as low as 0.1 mM having significant antioxidant effects. Ascorbic acid protected the RBC against hydrogen peroxide induced hemolysis as well (p less than 0.05). Ascorbic acid had a significant stimulatory effect on the rate of glucose oxidation by the pentose phosphate shunt (PPS), especially in the sickle RBC. Ascorbic acid did not protect the RBC from a patient with chronic hemolytic anemia due to G6PDTorrance from Heinz body formation, suggesting that an intact PPS is necessary for ascorbic acid to express its antioxidant properties. These data suggest that clinical trials should be undertaken to examine the efficacy of ascorbic acid in the treatment of SCD.
作者研究了抗氧化剂预防体外氧化对镰状红细胞（RBC）造成损伤的能力。1毫摩尔的抗坏血酸和α-巯基丙酰甘氨酸在与乙酰苯肼孵育期间能显著（p<0.005）防止RBC形成海因茨小体，而半胱氨酸、半胱胺和蛋氨酸则不能。抗坏血酸的作用呈浓度依赖性，低至0.1 mM的浓度也具有显著的抗氧化作用。抗坏血酸还能保护RBC免受过氧化氢诱导的溶血（p<0.05）。抗坏血酸对磷酸戊糖途径（PPS）氧化葡萄糖的速率有显著的刺激作用，尤其是在镰状RBC中。抗坏血酸不能保护因G6PDTorrance导致的慢性溶血性贫血患者的RBC免于形成海因茨小体，这表明完整的PPS对于抗坏血酸发挥其抗氧化特性是必要的。这些数据表明，应该进行临床试验以检验抗坏血酸治疗镰状细胞病（SCD）的疗效。
