Vesell E S, Shively C A
Science. 1974 Apr 26;184(4135):466-8. doi: 10.1126/science.184.4135.466.
A high pressure liquid chromatographic assay was developed to measure warfarin concentrations in biological fluids. Twelve healthy, unrelated volunteers received a single oral dose of warfarin (0.75 mg per kilogram of body weight). The mean plasma warfarin half-life was 36.3 +/- 3.5 hours by liquid chromatography but 55.9 +/- 8.4 hours by a currently used fluorimetric assay that fails to separate warfarin from its metabolites. Interindividual variation was greater and each half-life longer by the fluorimetric than by the chromatographic procedure. Warfarin shows less interindividual variation than that observed for other drugs primarily metabolized by hepatic microsomal mixed function oxidases. Advantages of specificity, rapidity, sensitivity, accuracy, and simplicity recommend liquid chromatography in the development of other drug assays.
开发了一种高压液相色谱分析法来测定生物体液中的华法林浓度。12名健康、无亲缘关系的志愿者单次口服华法林（每公斤体重0.75毫克）。通过液相色谱法测得的血浆华法林平均半衰期为36.3±3.5小时，而通过目前使用的未能将华法林与其代谢产物分离的荧光分析法测得的半衰期为55.9±8.4小时。荧光分析法的个体间差异更大，且每个半衰期都比色谱分析法长。与主要由肝微粒体混合功能氧化酶代谢的其他药物相比，华法林的个体间差异较小。特异性、快速性、灵敏性、准确性和简易性等优点使得液相色谱法在开发其他药物分析方法时具有优势。
