Moore M, Williams D E
Br J Cancer. 1972 Apr;26(2):90-8. doi: 10.1038/bjc.1972.14.
The immunogenicities of 15 murine osteosarcomata induced with a bone seeking radioisotope ((90)Sr) in normal and chimaeric CBA mice were studied. Attempts were made to induce tumour-specific immunity in syngeneic mice by treatment with x-irradiated (15,000 rad) tumour or surgical excision of developing subcutaneous tumour grafts. Resistance was evoked against 6 tumours and this was relatively weak. With the remaining tumours, no resistance against the immunizing tumour could be demonstrated, even though the transplantation tests were made highly sensitive by the use of inocula of as few as 2 × 10(3) cells in pre-irradiated (400 rad) hosts. Sera from mice immunized against each of the tumours were tested against viable cells of the immunizing tumour by indirect immunofluorescence. In no instance did tumour antisera give a convincing reaction with tumour cells although alloantisera raised by hyperimmunization of H-2 identical and H-2 different donors with osteosarcomata consistently gave strongly positive reactions. The results are interpreted as illustrating the weak tumour specific antigenicity of radiation-induced murine osteosarcomata. The possibility that antigenic deficiency is a consequence of immunosurveillance in this tumour system is discussed.
研究了在正常和嵌合CBA小鼠中，用亲骨性放射性同位素（锶90）诱导产生的15种小鼠骨肉瘤的免疫原性。尝试通过用X射线照射（15,000拉德）肿瘤或手术切除皮下生长的肿瘤移植物，在同基因小鼠中诱导肿瘤特异性免疫。对6种肿瘤产生了抵抗力，但相对较弱。对于其余的肿瘤，即使通过在预先照射（400拉德）的宿主中使用低至2×10³个细胞的接种物使移植试验高度敏感，也未能证明对免疫肿瘤有抵抗力。通过间接免疫荧光法，检测了针对每种肿瘤免疫的小鼠血清与免疫肿瘤的活细胞的反应。尽管通过用骨肉瘤对H-2相同和H-2不同的供体进行超免疫产生的同种抗血清始终产生强阳性反应，但肿瘤抗血清在任何情况下都未与肿瘤细胞产生令人信服的反应。结果被解释为说明辐射诱导的小鼠骨肉瘤的肿瘤特异性抗原性较弱。讨论了抗原缺陷是该肿瘤系统中免疫监视结果的可能性。
