通过疏水色谱法进行酶的纯化：从小麦胚芽中纯化天冬氨酸转氨甲酰酶的一种示例方法
Enzyme purification by hydrophobic chromatography: an alternative approach illustrated in the purification of aspartate transcarbamoylase from wheat germ.
作者信息
Yon R J
出版信息
Biochem J. 1974 Jan;137(1):127-30. doi: 10.1042/bj1370127.
Abstract
Two adsorbents containing similar numbers of hydrocarbon (C(10)) chains but different numbers of carboxyl groups were made by chemical modification of Sepharose. The use of these adsorbents to purify proteins, under conditions where hydrophobic adsorption is partly resisted by electrostatic repulsion, is illustrated in the purification of aspartate transcarbamoylase (EC 2.1.3.2) from wheat germ.
摘要
通过对琼脂糖进行化学修饰，制备了两种含有相似数量烃（C(10)）链但羧基数量不同的吸附剂。在疏水吸附部分受到静电排斥作用的条件下，利用这些吸附剂纯化蛋白质的过程，在从小麦胚芽中纯化天冬氨酸转氨甲酰酶（EC 2.1.3.2）的过程中得到了体现。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides.通过卤化氰将肽和蛋白质与多糖进行化学偶联。
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
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Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
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Chromatography of lipophilic proteins on adsorbents containing mixed hydrophobic and ionic groups.在含有混合疏水基团和离子基团的吸附剂上对亲脂性蛋白质进行色谱分析。
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Chromatography of plant aminoacyl-tRNA synthetases on omega-aminoalkyl sepharose columns.植物氨酰-tRNA合成酶在ω-氨基烷基琼脂糖柱上的色谱分析。
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Affinity chromatography of serum albumin with fatty acids immobilized on agarose.用固定在琼脂糖上的脂肪酸对血清白蛋白进行亲和层析。
J Biol Chem. 1973 Apr 10;248(7):2447-51.
8
Protein binding by agarose carrying hydrophobic groups in conjunction with charges.通过带有疏水基团并结合电荷的琼脂糖进行蛋白质结合。
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Biochem Biophys Res Commun. 1972 Oct 17;49(2):383-90. doi: 10.1016/0006-291x(72)90422-6.
10
Wheat-germ aspartate transcarbamoylase. Kinetic behaviour suggesting an allosteric mechanism of regulation.小麦胚芽天冬氨酸转氨甲酰酶。表明存在变构调节机制的动力学行为。
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