以“两性电解质”为固定相的生物特异性洗脱色谱法。
Biospecific-elution chromatography with 'imphilytes' as stationary phases.
作者信息
Yon R J
出版信息
Biochem J. 1977 Feb 1;161(2):233-7. doi: 10.1042/bj1610233.
Abstract
Six out of seven enzymes tested (four of them nicotinamide nucleotide-dependent dehydrogenases) showed differences in chromatographic behaviour in the presence and absence of their biospecific ligands, when chromatographed on immobilized amphipathic ampholytes ('imphilytes') as stationary phases. Some enzymes were adsorbed more tightly, others less tightly, in the presence of ligands. These results have implications for enzyme purification in general, and for some types of affinity chromatography in particular.
摘要
在以固定化两亲性两性电解质（“两性电解质”）作为固定相进行色谱分析时，所测试的七种酶中有六种（其中四种是烟酰胺核苷酸依赖性脱氢酶）在存在和不存在其生物特异性配体的情况下，色谱行为表现出差异。在配体存在的情况下，一些酶吸附得更紧密，另一些则吸附得较松散。这些结果总体上对酶的纯化有影响，特别是对某些类型的亲和色谱法有影响。
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