从丝衣霉子囊中制备游离耐热性子囊孢子。
Preparation of free heat-resistant ascospores from Byssochlamys asci.
作者信息
Michener H D, King A D
出版信息
Appl Microbiol. 1974 Apr;27(4):671-3. doi: 10.1128/am.27.4.671-673.1974.
Abstract
When Byssochlamys is grown for production of ascospores, some of the asci break up into their constituent ascospores, whereas others do not. For heat resistance studies, it is desirable to prepare a uniform suspension of free ascospores. This was accomplished with the aid of a pressure cell, from which a suspension of asci under high pressure was released to atmospheric pressure through a small orifice. Spores so treated had about the same heat resistance as untreated spores.
摘要
当培养烟曲霉以生产子囊孢子时，一些子囊会分裂成其组成的子囊孢子，而另一些则不会。对于耐热性研究，制备均匀的游离子囊孢子悬浮液是很有必要的。这借助于一个压力室来完成，在压力室中，高压下的子囊悬浮液通过一个小孔释放到大气压力下。如此处理过的孢子与未处理的孢子具有大致相同的耐热性。
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