表面活性剂的临界胶束浓度与其对细菌脱甲基酶的影响之间的相关性。

Correlation of the critical micelle concentrations of surfactants with their effects on a bacterial demethylase.

作者信息

Pinto J T, Frisell W R

出版信息

Proc Soc Exp Biol Med. 1975 Apr;148(4):981-5. doi: 10.3181/00379727-148-38672.

Abstract

The activity of a sarcosine dehydrogenase isolated from a strain of Pseudomonas is enhanced by the addition of Triton X-100, Brij 35, and Tween 80, and is inhibited by deoxycholate and Sarkosyl NL-97. 2,6-Dichlorophenolindophenol, which is used as the oxidant in the dehydrogenase assay, has also been employed as an indicator in the spectrophotometric determination of the critical micelle concentrations (CMC) of both the nonionic and anionic detergents under conditions optimal for the enzyme analyses. A correlation between the activation or inhibitory activities of the surfactants and their CMC values has been established.

摘要

从一株假单胞菌中分离出的肌氨酸脱氢酶的活性，在添加 Triton X - 100、Brij 35 和吐温 80 后会增强，而在添加脱氧胆酸盐和 Sarkosyl NL - 97 后会受到抑制。在脱氢酶测定中用作氧化剂的 2,6 - 二氯酚靛酚，也被用作分光光度法测定非离子和阴离子洗涤剂临界胶束浓度（CMC）的指示剂，测定条件为最适合该酶分析的条件。已建立了表面活性剂的活化或抑制活性与其 CMC 值之间的相关性。