Sprandel U, Hubbard A R, Chalmers R A
Res Exp Med (Berl). 1979 Jul 20;175(3):239-45. doi: 10.1007/BF01851280.
Resealed erythrocyte ghosts have been proposed as in vivo carriers for enzyme replacement therapy of inherited metabolic diseases. In comparative studies of methods for reversible hypotonic haemolysis of erythrocytes, a five-fold increased entrapment of human serum albumin was obtained by use of a dialysis procedure instead of direct dilution. The percentage incorporation of protein was also affected by varying mixing procedure, haematocrit, lysis, and resealing times but not by varying buffer composition or added protein concentrations over a wide range. Higher protein entrapment was observed with time-expired blood compared to fresh blood and this may be ascribed to increased osmotic fragility of the erythrocyte membrane in stored cells. Haemolysed and resealed ghosts prepared by any method used were smaller than normal erythrocytes as measured with a Coulter Counter and with a fluorescence-activated cell sorter, and protein entrapment reduced the ghost size further.
重新封闭的红细胞血影已被提议作为遗传性代谢疾病酶替代疗法的体内载体。在红细胞可逆性低渗溶血方法的比较研究中，通过使用透析程序而非直接稀释，人血清白蛋白的包封率提高了五倍。蛋白质的掺入百分比也受混合程序、血细胞比容、裂解和重新封闭时间的影响，但不受缓冲液组成变化或在很宽范围内添加的蛋白质浓度的影响。与新鲜血液相比，过期血液中观察到更高的蛋白质包封率，这可能归因于储存细胞中红细胞膜的渗透脆性增加。用库尔特计数器和荧光激活细胞分选仪测量，通过任何使用的方法制备的溶血和重新封闭的血影都比正常红细胞小，并且蛋白质包封进一步减小了血影大小。
