Hubbard A R, Sprandel U, Chalmers R A
Biochem J. 1980 Sep 15;190(3):653-8. doi: 10.1042/bj1900653.
The transport of organic acids across the membrane of resealed haemoglobin-containing erythrocyte 'ghosts' prepared by a dialysis technique has been studied. The present work forms part of studies directed towards the use of erythrocyte cellular carriers in enzyme-replacement therapy of inherited metabolic diseases. Oxalic acid, glycollic acid and glyoxylic acid were taken as representative of aliphatic acids of low molecular mass and benzoic and cinnamic acids as representative of unsubstituted aromatic acids. These selected acids are important in the diseases with which the present work is concerned. Comparison of influx and efflux transport characteristics showed that erythrocyte 'ghosts' retain transport properties closely similar to those of normal erythrocytes. Rapid transport was observed with all organic acids studied and there was a linear relationship between initial amount of influx and external concentration of aliphatic acid. Saturation of the transport system was not observed up to 1 mM external concentration, and the presence of plasma in the external medium had no effect on transport characteristics. Transport in intact erythrocytes and prepared erythrocyte 'ghosts' from patients with hyperoxaluria was also studied.
通过透析技术制备的含血红蛋白的红细胞“血影”膜上有机酸的转运已被研究。目前的工作是旨在将红细胞细胞载体用于遗传性代谢疾病酶替代疗法研究的一部分。草酸、乙醇酸和乙醛酸被用作低分子质量脂肪族酸的代表，苯甲酸和肉桂酸被用作未取代芳香族酸的代表。这些选定的酸在所关注的疾病中很重要。流入和流出转运特性的比较表明，红细胞“血影”保留了与正常红细胞非常相似的转运特性。在所研究的所有有机酸中都观察到了快速转运，脂肪族酸的初始流入量与外部浓度之间存在线性关系。在外部浓度高达1 mM时未观察到转运系统的饱和，外部介质中血浆的存在对转运特性没有影响。还研究了高草酸尿症患者完整红细胞和制备的红细胞“血影”中的转运。
