Jausel-Hüsken S, Deuticke B
J Membr Biol. 1981;63(1-2):61-70. doi: 10.1007/BF01969446.
Resealed human erythrocyte ghosts are regarded as valuable tools for the study of membrane properties. In order to investigate to what extent preparation procedures affect the yield of ghosts, their general properties, and their permeability, ghosts prepared by lysis at low (hypotonic media) and high (isotonic media) ionic strength were compared with each other and with native erythrocytes. For isotonic lysis, cells were either subjected to dielectric breakdown or suspended in isotonic NH4Cl solutions. In spite of very different characteristics of the lysis and the resealing process in the three types of preparations, the resulting ghosts do not differ in a number of features except for somewhat varying yields and for properties resulting from the mode of lysis. Specific transport properties, as characterized by the mediated fluxes of m-erythritol, L-arabinose, L-lactate, and sulfate, proved to be unaltered with a few unsystematic exceptions. The simple nonmediated fluxes of all these permeants, as measured in the presence of inhibitors, however, were enhanced between 1.5- and 4-fold, indicating a somewhat increased ground permeability (of the lipid domain) in all ghost membranes.
重新封闭的人红细胞血影被视为研究膜特性的宝贵工具。为了研究制备程序在多大程度上影响血影的产量、一般特性及其通透性，将在低（低渗介质）和高（等渗介质）离子强度下裂解制备的血影相互比较，并与天然红细胞进行比较。对于等渗裂解，细胞要么经受介电击穿，要么悬浮在等渗氯化铵溶液中。尽管三种制备方法中的裂解和重新封闭过程具有非常不同的特点，但除了产量略有不同以及裂解方式导致的特性有所不同外，所得血影在许多特征上并无差异。以间赤藓糖醇、L-阿拉伯糖、L-乳酸和硫酸盐的介导通量为特征的特定转运特性，除了一些无规律的例外情况外，证明没有改变。然而，在存在抑制剂的情况下测量的所有这些渗透物的简单非介导通量增加了1.5至4倍，表明所有血影膜中的（脂质结构域的）基础通透性略有增加。
