利用电子显微镜放射自显影技术对棘孢曲霉细胞壁中尼日利亚菌素的细胞内定位研究
Intracellular localization of nigeran in the wall of Aspergillus aculeatus by autoradiography with the electron microscope.
作者信息
Gold M H, Larson S, Segel I H, Stocking C R
出版信息
J Bacteriol. 1974 Jun;118(3):1176-8. doi: 10.1128/jb.118.3.1176-1178.1974.
Abstract
Aspergillus aculeatus mycelium was incubated with [(3)H]glucose under conditions that promote nigeran (mycodextran) accumulation (low pH, in the absence of nitrogen). Autoradiography revealed that essentially all the (3)H was localized around the hyphal perimeter. The results strongly support a hyphal wall location for nigeran.
摘要
将棘孢曲霉的菌丝体与[³H]葡萄糖在促进黑曲霉聚糖（霉菌葡聚糖）积累的条件下（低pH，无氮）进行培养。放射自显影显示，基本上所有的³H都定位于菌丝周边。这些结果有力地支持了黑曲霉聚糖位于菌丝壁的观点。
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