Suvatte V, Longsaman M
Southeast Asian J Trop Med Public Health. 1979 Mar;10(1):7-12.
Examination of the buffy coat preparation is a simple, rapid diagnostic aid for the early diagnosis of dengue hemorrhagic fever (DHF). The presence of many transformed lymphocytes, usually 20-50 per cent during the first few days of fever is a unique finding especially during a secondary infection. Similar findings are absent during the early phase of illness in other viral infections. The buffy coat appearance in DHF is also clearly different from those in patients with bacterial infections, which show many polymorphonuclear leukocytes containing toxic granules and vacuoles. The early buffy coat diagnosis in 320 cases of DHF correlated well with the later serologic diagnosis. The buffy coat diagnosis of secondary DHF was correct in 94.2% of cases as early as the third day of fever. This technique has proven to be clinically useful for early screening test of secondary DHF in Southeast Asian countries. The observation of many transformed lymphocytes in the peripheral blood of DHF patients also offer some insight into the pathogenesis of this condition.
检测血沉棕黄层制备物是登革出血热（DHF）早期诊断的一种简单、快速的诊断辅助方法。出现许多转化淋巴细胞，通常在发热的头几天占20% - 50%，这是一个独特的发现，尤其是在二次感染期间。在其他病毒感染的疾病早期阶段没有类似发现。DHF的血沉棕黄层表现也明显不同于细菌感染患者，细菌感染患者表现为许多含有毒性颗粒和空泡的多形核白细胞。对320例DHF患者的早期血沉棕黄层诊断与后期血清学诊断相关性良好。二次DHF的血沉棕黄层诊断在发热第三天时，94.2%的病例是正确的。这项技术已被证明在东南亚国家对二次DHF的早期筛查试验具有临床实用性。观察DHF患者外周血中许多转化淋巴细胞也为这种疾病的发病机制提供了一些见解。
