Brozović B, Purcell Y
J Clin Pathol. 1974 Mar;27(3):222-5. doi: 10.1136/jcp.27.3.222.
An automated micromethod for measuring iron concentration in serum employing thioglycollic acid and bathophenantroline sulphonate as reducing agent and chromogen respectively, is described. Measurements are carried out using an AutoAnalyzer (Technicon) and require 0.1 ml of sample. The reproducibility, assessed by the mean coefficient of variation (1.9%), and the mean recovery of iron added to samples (99.5%), as well as the correlation between the serum iron values estimated by the described method and the method recommended by the Expert Panel on Iron of the International Committee for Standardization in Haematology (r = 0.9779; p < 0.001; y = 0.9713 x -0.0546), is highly satisfactory. The method can also be used for measuring total ironbinding capacity of serum. The method appears equally suitable for routine work and research studies when a large number of samples and a small volume of serum are available.
本文描述了一种自动微量方法，该方法分别采用巯基乙酸和磺酸浴苯菲啰啉作为还原剂和显色剂来测定血清中的铁浓度。使用自动分析仪（Technicon）进行测量，所需样品量为0.1毫升。通过平均变异系数（1.9%）评估的重现性、添加到样品中的铁的平均回收率（99.5%），以及所描述方法估计的血清铁值与国际血液学标准化委员会铁专家小组推荐的方法之间的相关性（r = 0.9779；p < 0.001；y = 0.9713x - 0.0546），都非常令人满意。该方法也可用于测量血清的总铁结合能力。当有大量样品且血清体积较小时，该方法似乎同样适用于常规工作和研究。
