一种使用显色剂硝基-PAPS对血清铁进行灵敏、直接的比色测定法。
A sensitive, direct colorimetric assay of serum iron using the chromogen, nitro-PAPS.
作者信息
Makino T, Kiyonaga M, Kina K
机构信息
Department of Clinical Chemistry, Kanagawa Prefectural College of Nursing and Medical Technology, Yokohama, Japan.
出版信息
Clin Chim Acta. 1988 Jan 15;171(1):19-27. doi: 10.1016/0009-8981(88)90287-2.
A direct colorimetric method is presented for the determination of serum iron in 0.1-ml sized samples, using a new, water-soluble, reagent, 2-(5-Nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol Na salt (epsilon 585 nm = 9.4 X 10(4) l/mol per cm). Interference of copper and zinc in sera can be eliminated entirely by forming copper- and zinc-thioglycollate complexes immediately upon the dissociation of the protein-bound iron, copper and zinc by thioglycollate and sodium dodecyl sulfate. The serum blank was minimized by the use of sodium dodecyl sulfate as a protein denaturant. Within-run and between-run precision (CV) were in the range of 0.7-2.9% and 1.1-3.6%, respectively, depending on the serum iron content. A good correlation (r = 0.995) was obtained between this method and the reference method proposed by the International Committee for Standardization in Hematology.
本文介绍了一种直接比色法，用于测定0.1毫升大小样本中的血清铁，该方法使用一种新型水溶性试剂2-(5-硝基-2-吡啶偶氮)-5-(N-丙基-N-磺丙基氨基)苯酚钠盐（ε585 nm = 9.4×10⁴ l/mol·cm）。通过巯基乙酸盐和十二烷基硫酸钠使蛋白质结合的铁、铜和锌解离后，立即形成铜和锌的巯基乙酸盐络合物，可完全消除血清中铜和锌的干扰。使用十二烷基硫酸钠作为蛋白质变性剂可使血清空白降至最低。批内和批间精密度（CV）分别在0.7 - 2.9%和1.1 - 3.6%范围内，具体取决于血清铁含量。该方法与国际血液学标准化委员会提出的参考方法之间具有良好的相关性（r = 0.995）。
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