Dam R J, Burke C A, Griffith O H
Biophys J. 1974 Jun;14(6):467-72. doi: 10.1016/S0006-3495(74)85927-8.
The photoelectron quantum yields of 21 common amino acids and 15 polyamino acids were measured in the 180-240 nm wavelength region. On the average, the quantum yields of these two groups exhibit quite similar wavelength dependence. For lambda > 220 nm all amino acid and polyamino acid quantum yields are </=10(-7) electrons/(incident) photon. The mean yields increase to about 5 x 10(-7) electrons/photon at 200 nm and 5 x 10(-6) electrons/photon at 180 nm. L-tryptophan, L-tyrosine, and poly-L-tryptophan exhibit above average yields between 180 and 200 nm. Comparison with the dye phthalocyanine indicates that the quantum yield of the dye is two orders of magnitude greater than that of the amino acids from 200 to 240 nm, suggesting the feasibility of photoelectron labeling studies of biological surfaces.
在180 - 240纳米波长范围内测量了21种常见氨基酸和15种聚氨基酸的光电子量子产率。平均而言，这两组的量子产率表现出非常相似的波长依赖性。对于λ>220纳米，所有氨基酸和聚氨基酸的量子产率均≤10^(-7)电子/（入射）光子。平均产率在200纳米时增加到约5×10^(-7)电子/光子，在180纳米时增加到5×10^(-6)电子/光子。L-色氨酸、L-酪氨酸和聚-L-色氨酸在180至200纳米之间表现出高于平均水平的产率。与染料酞菁的比较表明，在200至240纳米范围内，染料的量子产率比氨基酸的量子产率高两个数量级，这表明对生物表面进行光电子标记研究是可行的。
