Haller E M, Brantner H
Zentralbl Bakteriol Orig A. 1979 Jun;243(4):522-7.
Since excenzymes are mainly involved in oncolysis by Cl. oncolyticum M 55 ATCC 13,732 the enzyme production of the strain should be examined under different growth conditions. The excretion of a protease and of two nucleases was observed in the nutrient medium for 72 h and the results obtained in a nitrogen atmosphere were compared with those obtained in an oxygen atmosphere. The cleavage of protein and of peptides was investigated by means of TLC. The importance of the exoenzymes for the oncolytic effect of Cl. oncolyticum M 55 was confirmed by animal experiments.
由于胞外酶主要参与溶瘤梭菌M 55 ATCC 13732的肿瘤溶解作用，因此应在不同生长条件下检测该菌株的酶产量。在营养培养基中观察蛋白酶和两种核酸酶72小时的分泌情况，并将在氮气环境中获得的结果与在氧气环境中获得的结果进行比较。通过薄层色谱法研究蛋白质和肽的裂解情况。动物实验证实了胞外酶对溶瘤梭菌M 55溶瘤作用的重要性。
