Lin C C, van de Sande H, Smink W K, Newton D R
Can J Genet Cytol. 1975 Mar;17(1):81-92. doi: 10.1139/g75-009.
Various factors involved in the production of "Q-bands" have been studied. It was found that a Zeiss standard WL fluorescent microscope required a shorter exposure time for photography as compared to a Zeiss photomicroscope. The minimal exposure time was obtained when the standard WL microscope was equipped with a UV light source containing a DC powered mercury burner and a concave mirror. Further, the pH and type of water used in the staining, washing and mounting of the slide were also important factors in producing clear and well differentiated "Q-bands". It also appears that the factors involved in the production of "Q-bands" effect the enhancement or quenching of fluorescence by poly d(A-T)-poly d(A-T) and salmon sperm DNA or poly dG-poly dC respectively. This preliminary report also suggests that DNA or polynucleotides with a specific base sequence may play an important role in Q-banding patterns on chromosomes.
对产生“Q带”的各种因素进行了研究。结果发现，与蔡司摄影显微镜相比，蔡司标准WL荧光显微镜拍照所需的曝光时间更短。当标准WL显微镜配备含直流供电汞灯和凹面镜的紫外光源时，可获得最短曝光时间。此外，玻片染色、洗涤和封固所用的水的pH值和类型也是产生清晰且区分良好的“Q带”的重要因素。还似乎产生“Q带”的因素分别影响聚d（A-T）-聚d（A-T）和鲑鱼精DNA或聚dG-聚dC对荧光的增强或淬灭。本初步报告还表明，具有特定碱基序列的DNA或多核苷酸可能在染色体的Q带模式中起重要作用。
