免疫球蛋白G的蛋白A与Fab和Fc片段的结合。
The binding of protein A of immunoglobulin G and of Fab and Fc fragments.
作者信息
Endresen C
出版信息
Acta Pathol Microbiol Scand C. 1979 Jun;87C(3):185-9.
Results are presented to show that protein A (pA) may fix to the Fab region of IgG outside the antigen binding site. Thus, pA-reactive Fab fragments were isolated both from specific anti-egg albumin and specific anti-measles virus antibodies. A probable significance of this Fab reactivity in the precipitation reaction between IgG and pA is discussed. Reactive IgG from human, guinea pig, rabbit, and porcine normal sera all showed similar avidity towards pA. Slight differences between IgG subclasses were, however, observed. The avidity of pA-reactive Fab and Fc fragments, isolated from normal porcine IgG, was highest in the latter.
结果表明，蛋白A（pA）可能结合于IgG抗原结合位点之外的Fab区域。因此，从特异性抗卵清蛋白和特异性抗麻疹病毒抗体中均分离出了与pA反应的Fab片段。本文讨论了这种Fab反应性在IgG与pA沉淀反应中的可能意义。来自人、豚鼠、兔和猪正常血清的反应性IgG对pA均表现出相似的亲和力。然而，观察到IgG亚类之间存在细微差异。从正常猪IgG中分离出的与pA反应的Fab和Fc片段，后者的亲和力最高。
Zotero 插件