Fc介导的免疫沉淀。I. IgG的Fc部分的新作用。
Fc-mediated immune precipitation. I. A new role of the Fc-portion of IgG.
作者信息
Møller N P
出版信息
Immunology. 1979 Nov;38(3):631-40.
By comparing the amounts of precipitate formed by the reaction between human serum albumin (HSA) and isomolar solutions of rabbit anti-HSA F(ab')2 fragments and of the corresponding intact rabbit anti-HSA IgG, respectively, it was found that the Fc portion of IgG was of great importance for the precipitin reaction. In the present antigen-antibody system, about half of the antigen was precipitated by this mechanism. This effect was called Fc-mediated precipitation and was most clearly expressed in the biologically important zone of low antigen excess and the zone of equivalence. It was found that the pepsin digestion did not change the ability of the F(ab')2 fragments to bind the antigen in comparison to intact IgG. Furthermore, turbidimetric analyses indicated that the Fc portion was involved in the overall mechanism of the precipitin reaction throughout the range of the precipitin curve.
通过分别比较人血清白蛋白（HSA）与等摩尔浓度的兔抗HSA F(ab')2片段溶液以及相应的完整兔抗HSA IgG反应形成的沉淀量，发现IgG的Fc部分对沉淀反应非常重要。在当前的抗原 - 抗体系统中，约一半的抗原通过这种机制沉淀。这种效应被称为Fc介导的沉淀，在低抗原过量的生物学重要区域和等价区域中表现最为明显。结果发现，与完整IgG相比，胃蛋白酶消化并未改变F(ab')2片段结合抗原的能力。此外，比浊分析表明，在沉淀曲线的整个范围内，Fc部分都参与了沉淀反应的整体机制。
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