Robbins J E, Hapner K D, Weber S D
Physiol Chem Phys. 1975;7(6):555-64.
The subunit molecular weight of glucose-6-phosphate dehydrogenase (G6PD) from baker's yeast has been evaluated. The subunit molecular weight value is shown to be 25,500 daltons by analytical ultracentrifugation, SDS-polyacrylamide gel electrophoresis, and the number of peptides produced by CNBr cleavage. The number of NADP binding sites was determined to be one per 25,500 dalton unit.
已对面包酵母中葡萄糖-6-磷酸脱氢酶(G6PD)的亚基分子量进行了评估。通过分析超速离心、SDS-聚丙烯酰胺凝胶电泳以及CNBr裂解产生的肽段数量,得出亚基分子量值为25,500道尔顿。确定每25,500道尔顿单位的NADP结合位点数量为一个。