Reilly K E, Allred J B
Department of Food Science and Technology, Ohio State University, Columbus 43210, USA.
Biochem Biophys Res Commun. 1995 Nov 22;216(3):993-8. doi: 10.1006/bbrc.1995.2718.
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using the highly specific periodate-digoxigenin antidigoxigenin method which is diagnostic for glycoproteins.
对从酿酒酵母中纯化得到的葡萄糖-6-磷酸脱氢酶(G6PD)的一种商业制剂,在非变性和变性条件下进行了聚丙烯酰胺凝胶电泳(PAGE)分析。通过活性染色和抗酵母G6PD抗体免疫印迹鉴定该酶,使用对糖蛋白具有诊断性的高特异性高碘酸盐-地高辛配基-抗地高辛配基方法,证明该酶含有碳水化合物。